Thanks Greg, I am looking for your Alkalinity Reagent preparation, refractor cal sln, or frankly Im interesting in test kit preps. Chemist in management misses the hood and rotovap>
Ah....the rotvap.....best part are the vapors coming off of it!
The alkalinity regeant can be prepared by dissolving a bit of bromophenol blue with some NaOH and then adding some HCl:
Generally:
0.2 g bromophenol blue
add a tiny bit of DI water, maybe 10 ml or so, then add 0.2 ml of 5 M NaOH. Once the bromophenol blue completely dissolves you can add 800 ml of DI water, then 4.15 ml of conc. HCl (the 36% stuff).
Then you take 15 ml of tank water, put it into a small plastic cup and add the alkalinity reagent dropwise until the blue goes away. You can calibrate the solution with a know alkalinity standard.
The refractometer soln. is as per Randy H-S's article:
made by dissolving 3.65 grams of sodium chloride in 96.35 grams (mL) of purified freshwater
The artificial saltwater (calcium/magnesium/alkalinity standard) is

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The EDTA prep for the HACH test kit modification is a little more involved. If you are really interested and already have the HA-4P test kit, let me know.